Thermal Image Processing System for the measurement of body temperature using a Drone

In December 2019, several cases of pneumonia caused by SARS-CoV-2 were identified in the city of Wuhan (China), which was declared by the WHO as a pandemic in March 2020 because it caused enormous problems to public health due to its rapid transmission of contagion. Being an uncontrolled case, precautions were taken all over the world to moderate the coronavirus that undoubtedly was very deadly for any person, presenting several symptoms, among them we have fever as a common symptom. A biosecurity measure that is frequently used is the taking of temperature with an infrared thermometer, which is not well seen by some specialists due to the error they present, therefore, it would not represent a safe measurement. In view of this problem, in this article a thermal image processing system was made for the measurement of body temperature by means of a drone to obtain the value of body temperature accurately, being able to be implemented anywhere, where it is intended to make such measurement, helping to combat the spread of the virus that currently continues to affect many people. Through the development of the system, the tests were conducted with various people, obtaining a more accurate measurement of body temperature with an efficiency of 98.46% at 1.45 m between the drone and the person, in such a way that if it presents a body temperature higher than 38° C it could be infected with COVID-19. © 2023 IEEE.

Chest X-ray Imaging System for Early Detection of Tuberculosis

Today it is observed that few people respect the biosecurity measures announced by the WHO, which aimed to reduce the amount of COVID-19 infection among people, even knowing that this virus has not disappeared from our environment, being an unprecedented infection in the world. It should be noted that before this pandemic, tuberculosis affected millions of people, having a great role because it is highly contagious and directly affects the lungs, although it has a cure, if it is not treated in time it can be fatal for the person, although there are many methods of detection of tuberculosis, one that is most often used is the diagnosis by chest x-ray, although it has low specificity, when the image processing technique is applied, tuberculosis would be accurately detected. In view of this problem, in this article a chest X-ray image processing system was conducted for the early detection of tuberculosis, helping doctors to detect tuberculosis accurately and quickly by having a second opinion by the system in the analysis of the chest x-ray, prevents fatal infections in patients. Through the development of the tuberculosis early detection system, it was possible to observe the correct functioning of the system with an efficiency of 97.84% in the detection of tuberculosis, detailing the characteristics presented by normal or abnormal images so that the doctor detects tuberculosis in the patient early. © 2023 IEEE.

Evaluation of animal biosafety webinar series for professionals working in animal facilities across Pakistan during the COVID-19 pandemic

Experimental research with animals can help the prevention, cure, and alleviation of human ailments. Animal research facilities are critical for scientific advancement, but they can also pose a higher risk than other biomedical laboratories. Zoonosis, allergic reactions, bites, cuts, and scratches by animals are all substantial concerns that can occur in animal facilities. Furthermore, human error and unexpected animal behavior pose a risk not just to humans, but also to the environment and the animals themselves. The majority of biosafety and biosecurity training programs focus on clinical and biomedical laboratories dealing with human safety factors, with little emphasis on animal biosafety. The current virtual training was designed to improve biosafety and biosecurity capabilities of animal laboratory personnel, researchers, and veterinarians from different regions of Pakistan. The results revealed that understanding was improved regarding triggers for risk assessment in addition to annual and regular reviews (56% to 69%), biosecurity (21% to 50%), decontamination (17% to 35%), safe handling of sharps (21% to 35%), Dual Use Research of Concern (DURC) (17% to 40%), Personal Protective Equipment (PPE) usage by waste handlers (60.9% to 75%), waste management (56% to 85%), animal biosafety levels (40.57% to 45%), and good microbiological practices and procedures (17% to 35%). To bring human and animal laboratories up to the same level in terms of biosafety and biosecurity, it is critical to focus on areas that have been overlooked in the past. Training programs focusing on animal biosafety should be conducted more frequently to strengthen bio risk management systems in animal research facilities.Copyright © 2021

Effect of changes of vaccination strategies on IBV epidemiology, diagnosis and control: an Italian retrospective study

Infectious bronchitis virus (IBV) is among the most impactful poultry pathogens, whose control, based on biosecurity and routine vaccination, is hampered by the existence of countless genetic variants sharing poor cross-protection. A retrospective study was conducted on IBV positive samples collected in Italian broiler farms from 2012 to 2019. In 2015, the adopted vaccination protocol shifted from a Mass and 793B-based vaccines to the administration of Mass and QX vaccines, allowing to study how changes in vaccination strategies may affect IBV epidemiology, control and diagnosis in the field. The most frequently detected lineages were QX (70.3%), 793B (15.8%) and Mass (11.9%). The relative frequencies of QX and 793B detections remained stable throughout the study, while Mass detections significantly increased after the vaccination change. Rather than to an actual growth of Mass population size, this finding may be attributable to different vaccine interactions, with Mass strains being more frequently concealed by 793B vaccines than by QX ones. Based on the obtained results, the two vaccination protocols appear to be similarly effective in fighting IB outbreaks, which in the last decade have been caused primarily by QX field strains in Italy. These results indicate that vaccination strategies may significantly affect IBV epidemiology and diagnosis, and should therefore be considered when choosing and interpreting diagnostic assays and planning control measures.

Ethical and social insights into synthetic biology: predicting research fronts in the post-COVID-19 era.

As a revolutionary biological science and technology, synthetic biology has already spread its influence from natural sciences to humanities and social sciences by introducing biosafety, biosecurity, and ethical issues to society. The current study aims to elaborate the intellectual bases and research front of the synthetic biology field in the sphere of philosophy, ethics, and social sciences, with knowledge mapping and bibliometric methods. The literature records from the Social Sciences Citation Index and Arts & Humanities Citation Index in the Web of Science Core Collection from 1982 to 2021 were collected and analyzed to illustrate the intellectual structure of philosophical, ethical, and social research of synthetic biology. This study profiled the hotspots of research focus on its governance, philosophical and ethical concerns, and relevant technologies. This study offers clues and enlightenment for the stakeholders and researchers to follow the progress of this emerging discipline and technology and to understand the cutting-edge ideas and future form of this field, which takes on greater significance in the post-COVID-19 era.

Applications of the One Health concept: Current status in the Middle East

Background: The One Health concept (OHC) seeks to improve the health of plants, animals, and humans because improving animal and plant health will increase the capacity for improving human health. Many risks such as plant and animal biotechnology applications have the potential to generate new diseases that can be transmitted to humans. In this way, the health of humans, animals, and plants is interrelated and depends on one another. However, it has been difficult to apply the OHC in some countries, such as those in the Middle East. The absence of financial support in the region is a major hindrance to applying this concept in the region. The application of the OHC requires the support of specialists who can advocate the government for support in launching OHC-related projects. Here, we discuss the OHC in the context of antimicrobial resistance, zoonotic diseases, and biosafety/biosecurity, which are important public health issues. Furthermore, we describe the current status of the OHC in the Middle East and recent research conducted related to this concept. There has been recent international solidarity in the application of the OHC to reduce risks that threaten the health of organisms. Several countries jointly launched the Global Health Security Agenda in 2014 with the aim of realizing a world that is free of infectious disease-related health risks. However, no previous review articles have examined the applications of the OHC in the Middle East region. This article discusses the OHC in terms of its needs and current applications in the Middle East. Methodology: The following keywords were used in the search: "One Health," "Middle East," "medicinal plants," "viruses," "rabies," "MERS," and "antimicrobial resistance." Related papers were obtained by searching for these keywords using available search engines, such as PubMed, Google Scholar, and Google search, as well as international organization websites. Conclusion(s): The concept of One Health is relatively new and has not been applied in most countries, possibly because the value of this concept for improving human health is not well understood. The key principle defining this concept and its importance is the interdependency of plants, animals, and human health. By applying the OHC, humans can benefit from healthy plants and animals by enhancing their growing conditions, medications, and environments. This would in turn improve general human health by allowing the safe extraction of therapeutics and food resources.Copyright © 2023

Evaluation of Ultraviolet Type C Radiation in Inactivating Relevant Veterinary Viruses on Experimentally Contaminated Surfaces.

Many swine farms employ UVC treatment in employees' personal belongings and small tools entering farms as part of the biosecurity protocol to decrease the risk of pathogen introduction into the operation. However, the UVC efficacy in some veterinary viruses is not fully evaluated. This study evaluated the efficacy of ultraviolet type C (UVC) radiation in inactivating seven relevant veterinary viruses: Swine Poxvirus (SwPV), Porcine Reproductive and Respiratory Syndrome Virus (PRRSV), Porcine Epidemic Diarrhea Virus (PEDV), Swine Influenza Virus (SIV), Bovine Viral Diarrhea Virus (BVDV), Porcine Parvovirus (PPV), and Senecavirus A (SVA). The experimentally contaminated materials included polystyrene and filter paper. The samples were exposed to UVC for 5 min (total dose of 360 mJ/cm2). The UVC treatment caused a decrease over 4 log10 in SwPV titer on the polystyrene surface, whereas it consistently reduced about 5 log10 in PPV and SVA samples. No viable virus was recovered from PRRSV, PEDV, SIV, and BVDV samples. In filter paper, conversely, the efficacy was reduced. This study provides essential information on the inactivation effectiveness of a specific dose of UVC on important veterinary viruses, further supporting the rational application and strategic guidance for UVC radiation use to disinfect materials.

Zooanthropogenic potential of SARS-CoV-2 virus: Implications for vaccine-mediated immunity

The human pandemic caused by Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) that started in December, 2019 is still continuing in various parts of the world. The SARS-CoV-2 has evolved through sporadic mutations and recombination events and the emergence of alternate variants following adaptations in humans and human-to-animal transmission (zooanthraponosis) has raised concerns over the efficacy of vaccines against new variants. The animal reservoir of SARS-CoV-2 is unknown despite reports of SARS-CoV- 2-related viruses in bats and pangolins. A recent report of back-andforth transmission of SARS-CoV-2 between humans and minks on mink farms in the Netherlands has sparked widespread interest in zooanthroponotic transmission of SARS-CoV-2 followed by reemergence to infect human populations. The risk of animal to human transmission depends on virus-host interaction in susceptible species that may be short-term or long term risks. The short term risk might be due to infection to humans during the viremic stage in susceptible animals. The long term risk might be either due to persistence of the virus at population level or latency of infection leading to risk of evolution and re-emergence of the virus. Experimental studies have identified a range of animals that are susceptible and permissive to SARS-CoV-2 infection viz. cats, ferrets, hamsters, mink, non-human primates, tree shrews, raccoon dogs, fruit bats, and rabbits. The health impacts of SARS-CoV-2 infection in animals are unknown and it is likely that other susceptible species have not been discovered yet. Apart from farmed animals, stray cats and rodents have been identified as a potential opportunity for ongoing transmission in intense farming situations. Recognizing animal species that are most susceptible to infection is the first step in preventing ongoing transmission from humans. Minimizing the risk of zooanthraponosis requires multi-sectoral coordination that includes implementation of strict biosecurity measures such as controlled access to farms that house susceptible animals, bio-secure entry and exit protocols, disinfection protocols in farm, down time for animal transport vehicles and daily assessments of human handlers for exposure to SARS-CoV- 2. Hence, active surveillance in animal species that are prioritized based on risk assessment need to be initiated in coordination with health and environment sectors for early identification of emerging and re-emerging variants of SARS-CoV-2 virus in animals.

Global Veterinary Diagnostic Laboratory Equipment Management and Sustainability and Implications for Pandemic Preparedness Priorities1.

Substantial investments into laboratories, notably sophisticated equipment, have been made over time to detect emerging diseases close to their source. Diagnostic capacity has expanded as a result, but challenges have emerged. The Equipment Management and Sustainability Survey was sent to the Veterinary Services of 182 countries in mid-2019. We measured the status of forty types of laboratory equipment used in veterinary diagnostic laboratories. Of the 68,455 items reported from 227 laboratories in 136 countries, 22% (14,894/68,455) were improperly maintained, and 46% (29,957/65,490) were improperly calibrated. Notable differences were observed across World Bank income levels and regions, raising concerns about equipment reliability and the results they produce. Our results will advise partners and donors on how best to support low-resource veterinary laboratories to improve sustainability and fulfill their mandate toward pandemic prevention and preparedness, as well as encourage equipment manufacturers to spur innovation and develop more sustainable products that meet end-users' needs.

Biosafety Protocols in Dental Care: a Bibliographical Review

The biosafety protocols in dental care in the face of the COVID-19 pandemic, in medical offices in Los Ceibos sector of Ibarra city during the period October 2021 to March 2022 had deficiencies, they do not have enough information about their application. The stated objective was the biosafety protocol's evaluation for dental care in the Los Ceibos sector. In this research, methods of the theoretical level have been used;as well as the empirical level, which are applied with their respective instruments, such as the interview guide and the questionnaire. With this preliminary result, it is concluded that the knowledge of the inhabitants about these protocols is regular. At the doctor's office does not possess all of the level of requisite information to achieve the effectiveness of the protocols so that a revision of the existent bibliography prevails over the theme to complement its implementation. 23 documentary sources was consulted, 18 of them in Spanish and 5 in English among primary sources and secondaries as well as another one back up acceded by means of the principal managers of quest.

Development of a Novel in-Vitro Hcov-Oc43 Screening Method

Aim: To develop a simple, inexpensive antiviral screening assay, applicable to SARS-CoV-2, using a plate-based bioassay approach to assess the in-vitro activity of compounds against HCoV-OC43. Background(s): Despite the successful deployment of vaccines against SARS-CoV-2 there remains a need for effective antivirals for acute infection treatment. A distinct problem facing the search for new anti-coronavirus compounds is the cost of antiviral screening, compounded by the biosecurity concerns of live SARSCoV- 2 culture. In concert with low pathogenic surrogate virus use, the resazurin reduction assay, which is often employed for compound cytotoxicity assessments can be employed for safe, rapid and inexpensive antiviral screening. Method(s): In-vitro cell based resazurin reduction assays were optimised using remdesivir as a control compound for the assessment of anti-HCoV-OC43 activity. Following optimisation, 246 purified natural compounds from the University of Western Australia's compound collection,were screened using the resazurin bioassay as a primary screen, under pre-treatment and cotreatment conditions. Five compounds, which demonstrated anti- HCoV-OC43 activity, were chosen for secondary screening with dose responses determined using qRT-PCR. Result(s): Primary screens of the 246 compounds using the resazurin bioassay identified five compounds with a relative viral inhibition >60% and a relative cell viability >70% (Table 1). The Z factor of the pre-treatment and co-treatment assays was >0.5 (average +/- SD;0.85 +/- 0.07, 0.91 +/- 0.03 respectively). Further dose response analysis of the top five compounds identified one compound with an IC50 value <10 muM. Conclusion(s): The method developed is an appropriate primary screening tool for the identification of novel compounds with anti-HCoV-OC43 activity.Copyright © 2023 Southern Society for Clinical Investigation.

Responding to Future Pandemics: Biosecurity Implications and Defense Considerations

In an evolv ing and expanding biothreat landscape caused by emerging biotechnologies, increases in global infectious disease outbreaks, and geopolitical instability, the Department of Defense now faces challenges that alter its traditional approach to biothreats and prompt the need for modernized, improved preparedness for--and response to--potential biothreat scenarios. These challenges further complicate specif ic weaknesses revealed by the COVID-19 pandemic, including the Department's inability to sustain the military mission while meeting intragovernmental expectations to assist with civilian public health resources and services. [ FROM AUTHOR] Copyright of Parameters: U.S. Army War College is the property of U.S. Army War College and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full . (Copyright applies to all s.)

Probable Transmission of SARS-CoV-2 from African Lion to Zoo Employees, Indiana, USA, 2021.

We describe animal-to-human transmission of SARS-CoV-2 in a zoo setting in Indiana, USA. A vaccinated African lion with physical limitations requiring hand feeding tested positive for SARS-CoV-2 after onset of respiratory signs. Zoo employees were screened, monitored prospectively for onset of symptoms, then rescreened as indicated; results were confirmed by using reverse transcription PCR and whole-genome virus sequencing when possible. Traceback investigation narrowed the source of infection to 1 of 6 persons. Three exposed employees subsequently had onset of symptoms, 2 with viral genomes identical to the lion's. Forward contact tracing investigation confirmed probable lion-to-human transmission. Close contact with large cats is a risk factor for bidirectional zoonotic SARS-CoV-2 transmission that should be considered when occupational health and biosecurity practices at zoos are designed and implemented. SARS-CoV-2 rapid testing and detection methods for big cats and other susceptible animals should be developed and validated to enable timely implementation of One Health investigations.

Simulation Model of the Effect of the COVID-19 Biosecurity Protocol in the Queues for the Return to Face-to-Face Classes.

This article presents a simulation model of the effect of the COVID-19 Biosafety protocol in the Queues for the return to face-to-face classes as a basis for the return of educational activities. The restrictive measures to face the COVID-19 pandemic, greatly affected the normality of the development of activities carried out in higher education, constituting a great challenge since the measures and conditions of return and access to institutions are different from what was previously carried out before the pandemic. The objective of this article is to carry out a simulation of queues through SIMIO (Logistics process simulation software), where the number of students expected per day and the average entry times are taken into account to adjust them to an exponential distribution, from this information loaded into the simulation it is They will determine if the times increase or decrease taking into account the theory of queues and conditions presented in the study such as the probability distribution of the times of each of the processes involved in the process of accessing the University facilities.

Engaged learning: lessons learned by subject-matter experts from COVID-19 in the U.S. swine industry

When industries fail to address warnings adequately, risk situations may manifest into crises. Although the U.S. swine industry planned thoroughly for potential infectious disease outbreaks among the animal population, COVID-19 highlighted a planning gap: What happens if the humans working to keep the industry operational can no longer work? This oversight ultimately resulted in a crisis event impacting all aspects of the U.S. swine industry. This study examines the process of engaged learning from failure that took place via retrospective accounts provided in interviews with 18 industry subject-matter experts. Lessons learned, practical applications, and areas for future research suggest that engaged learning values diverse perspectives and, ultimately, promotes meaningful systemic change. Conversely, organizations opting to disengage during or after crises are likely to repeat mistakes and experience similar crises in the future. © 2023 National Communication Association.

Oral and Maxillofacial Radiology During the Coronavirus Disease 2019 Pandemic: Recommendations for a Safer Practice

The Coronavirus 2019 disease (COVID-19), was declared a pandemic by the World Health Organization on March 11, 2020, and registered the first case in Costa Rica on March 6, 2020, and 105 days later reported 2058 cases, with a wide age range (0-92 years). Its etiologic agent is the beta coronavirus SARS-CoV-2 with confirmed human-to-human transmission, mainly by the respiratory route. The presence of asymptomatic carriers of the virus and its transmission even without the manifestation of symptoms challenges health professionals worldwide. For this reason, assuming all patients who need dental care as possible carriers of the SARS-CoV-2 is mandatory and consequently, applying biosecurity measures with strict criteria. In the present literature review, we aim to recommend biosecurity measures to be applied during imaging examinations of dental patients due to the Coronavirus-2019 pandemic. We found that in the absence of treatment for COVID-19 disease and because of its high transmissibility, the most effective measures to prevent its spread are those allowing its containment. Therefore, the authors recommend: 1) acquiring imaging exams just in case of dental urgencies or emergencies, 2) prioritizing the use of extraoral exams, such as panoramic radiography or CBCT, under the principle of ALADAI and 3) the intraoral exams should be used just in case of a lack of extraoral imaging devices, or because of the need to solve diagnostic tasks in which they have a superior diagnostic value than those of the extraoral examinations. © 2020, Universidad de Costa Rica. All rights reserved.

Access Control Through Mask Detection and Estimation of People Capacity in Covered Premises

The health emergency due to the COVID-19 pandemic requires the search for technological and intelligent solutions that facilitate the control of biosecurity measures such as social distancing, to use of a mask, and capacity in covered spaces. This work aims to develop a prototype based on artificial vision algorithms, capable of performing the automatic mask detection and people counting who go to covered premises such as bars, restaurants, gyms, cinemas, and micro-market among others. The prototype implements SSD-MobileNet object detection and SORT tracking algorithms that work on the electronic device NVIDIA Jetson Nano, equipped with two video cameras to perform mask detection and people counting respectively, as well as speakers, for emission of audible alert messages about the use of mask and the capacity estimation within the premise and an external web server too in which people counter information is displayed. © 2023, The Author(s), under exclusive license to Springer Nature Switzerland AG.

Successful Eradication of Porcine Epidemic Diarrhea in an Enzootically Infected Farm: A Two-Year Follow-Up Study.

Porcine epidemic diarrhea virus (PEDV) has negatively affected the welfare of animals and their productivity in South Korea for three decades. A shortage of effective control measures has led to the virus becoming endemic in domestic pig populations. This study aimed to describe how our intervention measures were implemented for PEDV elimination in an enzootically infected farm. We operated a risk assessment model of PEDV recurrence to obtain information about the virus itself, herd immunity, virus circulation, and biosecurity at the farm. Next, we conducted a four-pillar-based two-track strategy to heighten sow immunity and eradicate the virus, with longitudinal monitoring of immunity and virus circulation, involving strict biosecurity, prime-boost pre-farrow L/K/K immunization, all-in-all-out and disinfection practices in farrowing houses, and disinfection and gilt management in wean-to-finish barns. In particular, we observed a high prevalence and long-term survival of PEDV in slurries, posing a critical challenge to PED eradication and highlighting the necessity for consecutive testing of barn slurry samples and for the management of infected manure to control PEDV. Genetic analysis of PEDVs in this farm indicated that genetic drift continued in the spike gene, with a substitution rate of 1.683 × 10-4 substitutions/site/year. Our study underlines the need for active monitoring and surveillance of PEDV in herds and their environments, along with the coordinated means, to eliminate the virus and maintain a negative herd. The tools described in this study will serve as a framework for regional and national PED eradication programs.